상아질 접착제의 Bis-GMA 및 HEMA가 세포독성에 미치는 영향
Effects of Bis-GMA and HEMA on the cytotoxicity of dentin bonding agents
박현제, 손경미, 권지현, 박희철, 주정정, 양형철,
소속 상세정보
박현제 ( Park Hyun-Jae ) - 서울대학교 치의학대학원 치과생체재료과학교실
손경미 ( Son Kyung-Mi ) - 서울대학교 치의학대학원 치과생체재료과학교실
권지현 ( Kwon Ji-Hyung ) - 서울대학교 치의학대학원 치과생체재료과학교실
박희철 ( Park Hee-Chul ) - 서울대학교 치의학대학원 치과생체재료과학교실
주정정 ( Zhu Ting Ting ) - 서울대학교 치의학대학원 치과생체재료과학교실
양형철 ( Yang Hyeong-Cheol ) - 서울대학교 치의학대학원 치과생체재료과학교실
KMID : 0362220130400020143
Abstract
It has been reported that dentin bonding agents (DBA) used in composite resin adhesion have a strong cytotoxicity. Bisphenol A glycidyl methacylate (Bis-GMA) and 2-hydroxyethyl methacrylate (HEMA) are known as the main ingredients in a large number of resin-based DBA. In this study, we quantified Bis-GMA and HEMA in dentin bonding agents by using high performance liquid chromatography (HPLC), and investigated whether these resin monomers were responsible for the cytotoxicity of DBA. In 1 ㎎ of Adper Single Bond 2, Clearfil-SE Bond Adhesive and Adper Scotchbond Multi-Purpose Adhesive, 0.3475, 0.4899 and 0.2717 ㎎ of Bis-GMA were detected, respectively, and 0.1475, 0.3092 and 0.4372 ㎎ of HEMA were detected, respectively. Based on the results of the cytotoxicity, Bis-GMA is likely a major cause of cytotoxicity. In 1㎎ of Clearfil-SE primer, Adper Scotchbond Multi-Purpose Primer, One-UP Bond F plus A and One-UP Bond F plus B, 0.1377, 0.6099, 0.1024 and 0.8595 ㎎ of HEMA were detected respectively. Bis-GMA was not detected in those DBA (detection limit: 5 ㎛). Based on the results of the cytotoxicity, the cytotoxicity of Clearfil-SE Bond Primer is expected due to HEMA. Adper Scotchbond Multi-Purpose Primer showed low cytotoxicity despite the high amount of HEMA. Therefore, it is assumed that the components that exhibit the antagonic effect on the cytotoxicity of HEMA are included in Adper Scotchbond Multi-Purpose Primer. The result of One-UP Bond F plus B showed that HEMA is a major cause of cytotoxicity. But, in case of One-UP Bond F plus A, other cytotoxic ingredients rather than HEMA likely exist. Bis-GMA and HEMA was not detected in Prime & Bond NT, I Bond and G-Bond, in which the other cytotoxic ingredient was assumed included.
키워드
Dentin bonding agent; Bis-GMA; HEMA; cytotoxicity; HPLC
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